Fig. 1

C3G ameliorated renal fibrosis in UUO mice. (A-B) PCR detection of α-SMA and fibronectin in sham-operated and UUO groups after treatment with C3G. (C) Protective effect of C3G against renal tissue damage detected by H&E and Masson and α-SMA immunohistochemistry (×400). Black arrows represented tubular necrosis, red arrows represented detachment of tubular epithelial cells, and green arrows represented interstitial fibrosis. (D) The quantification of tubular damage score by H&E staining. (E) The quantification of total collagen area by MASSON staining. (F) The quantification of of α-SMA expression by immunohistochemistry. Values were expressed as the mean ± SEM. *P < 0.05, relative to the sham group. #P < 0.05, relative to the UUO group.