Fig. 3
C3G attenuated TGF-β1-induced fibrosis in HK-2 cells. (A) HK-2 cells were treated with different concentration (50, 100, 150, 200µmol/L) of C3G for 24 h, and then cell viability was detected by CCK-8; (B) HK-2 cells were treated with different concentrations (50, 100, 150, 200µmol/L) of C3G and TGF-β1 (5ng/mL), and cell viability was detected by CCK-8. (C-D) α-SMA protein level was examined by western blot in different treatment groups, and the quantification was showed as relative to the sham-operated group. (E) α-SMA Immunofluorescence was detected in each group. Values were expressed as the mean ± SEM. *P < 0.05, relative to the control group. #P < 0.05, relative to the TGF-β1 group.
