Fig. 4
C3G ameliorated EMT and extracellular matrix deposition induced by TGF-β1 in HK-2 cells. (A-B) E-cadherin, Snail, vimentin and N-cadherin protein level were examined by western blot in different groups, and the quantification of E-cadherin, snail, vimentin and N-cadherin was showed as relative to the sham-operated group. (C) mRNA levels of E-cadherin, snail, vimentin and N-cadherin were detected by real-time RT-PCR in different group. (D) FN immunofluorescence was detected and quantified. (E-F) Col-I and Col-III protein level were examined by western blot in different groups, and the quantification was showed as relative to the sham-operated group. (G) mRNA levels of Col-I and Col-III were detected by real-time RT-PCR in different group. Values were expressed as the mean ± SEM. *P < 0.05, relative to the control group. #P < 0.05, relative to the TGF-β1 group.
