Fig. 5
NDRG2 was upregulated after UUO and TGF-β1 treatment. (A-C) NDRG2 protein and mRNA level were detected by western blot and RT-PCR at different period (3, 7 and 14 day) of UUO model establishment, and the quantification was showed as relative to the sham-operated group. (D) Immunohistochemical staining of NDRG2 was performed at different period (3, 7 and 14 day) of UUO model establishment. (E-G) NDRG2 protein and mRNA level were detected by western blot and RT-PCR at different period (6, 12, 24 h) of TGF-β1 treatment, and the quantification was showed as relative to the control group. (H) NDRG2 immunofluorescence was detected at different period (6, 12, 24 h) of TGF-β1 treatment. Values were expressed as the mean ± SEM. *P < 0.05, relative to the sham and control group.
