Table 1 Primer sequences and antibodies used in the present work.
From: RORα fine-tunes the circadian control of hepatic triglyceride synthesis and gluconeogenesis
Gene | Primer sequences | Antibody references | |||
|---|---|---|---|---|---|
Forward | Reverse | Proteintech | Santa-Cruz | Cell Signalling | |
PCK1 | CAACTTCGGCAAATACCTG | CTGTCTTCCCCTTCAATCC | 16754-1-AP | ||
GyK | GAAACTTCGTTGGCTCCTTG | CGTCCTGCTTGCATTTGTTA | 13360-1-AP | ||
PKLR | CGAAAAGCCAGTGATGTGGTGG | GATGCCATCGCTCACTTCTAGG | 22546-1-AP | ||
Srebf1 | CCGTCACTTCCAGCTAGACC | TGAGCCTCAGCTAGGGAAAA | 14088-1-AP | SC-366 | |
Insig1 | TCACAGTGACTGAGCTTCAGCA | TCATCTTCATCACACCCAGGAC | 55282-1-AP | ||
Insig2a | CCCTCAATGAATGTACTGAAGGATT | TGTGAAGTGAAGCAGACCAATGT | 24766-1-AP | ||
Insig2b | CCGGGCAGAGCTCAGGAT | GAAGCAGACCAATGTTTCAATGG | 24766-1-AP | ||
NR1f3 (RORγ) | TTTTGAGGATACCAGGCATC | CCACATCTCCCACATTGACTTC | 13205-1-AP | ||
NR1f1 (RORα) | GCACCTGACCGAAGACGAAA | GAGCGATCCGCTGACATCA | SC-28612 | ||
Nr1d1 (Reverbα) | AACCTCCAGTTTGTGTCAAGGT | GATGACGATGATGCAGAAGAAG | SC-393215 | ||
ACC | AGTGCCTTCCTCGGTTTCTT | GCCAGGATTCAAGGTGTGTT | #3662 | ||
FASN | ATCCCAGCACTTCTTGATGG | CCGAAGCCAAATGAGTTGAT | #3180 | ||
Bmal1 | CCACCTCAGAGCCATTGATACA | GAGCAGGTTTAGTTCCACTTTGTCT | 14268-1-AP | ||
Clock | ACCACAGCAACAGCAACAAC | GGCTGCTGAACTGAAGGAAG | 18094-1-AP | ||
Cry1 | CTGGCGTGGAAGTCATCGT | CTGTCCGCCATTGAGTTCTATG | |||
Per2 | TGTGCGATGATGATTCGTGA | GGTGAAGGTACGTTTGGTTTGC | |||
PPARα | GCACTGGAACTGGATGACAG | TTTAGAAGGCCAGGACGATCT | |||
LXRα | CGGGCTTCCACTACAATGTT | TCAGGCGGATCTGTTCTTCT | |||
Chrebpα | TTTTGACCAGATGCGAGACA | TGGCGTAGGGAGTTCAGG | |||
GAPDH | CAAGGAGTAAGAAACCCTGGACC | CGAGTTGGGATAGGGCCTCT | |||
36B4 | GCTGATGGGCAAGAACACCA | CCCAAAGCCTGGAAGAAGGA | |||
HPRT | AGGACCTCTCGAAGTGT | TCAAATCCCTGAAGTACTCAT | |||
