Fig. 2
From: CRISPR-Cas9 genetic screens reveal regulation of TMPRSS2 by the Elongin BC-VHL complex
TMPRSS2 KO affects SARS-CoV-2 entry into colon epithelial Caco-2-ACE2 cells. (A) Caco-2-ACE2 Cas9 cells stably expressing sgRNAs specific for b2m, ACE2 or TMPRSS2 were stained with ACE2- and TMPRSS2-specific antibodies or secondary antibody alone and analysed by flow cytometry. (B) Caco-2-ACE2 Cas9 cells from the panel 2A were infected with rSARS-CoV-2 Venus or Omicron BA.2 at an MOI of 0.1 or 1, fixed 24 h later and analysed by automated microscopy. Y-axis indicates percentage of GFP (rSARS-CoV-2 Venus) or N-protein (BA.2)-positive cells. Data are presented as mean of n = 3 biological replicates ± s.d. The statistical significance was assessed by two-way ANOVA and Bonferroni’s multiple comparison correction. See also Figures S2, S3 and S4.
