Fig. 6

Effects of (a) 24 h and (b) 48 h of deoxynivalenol (DON) and baicalin (BAI) treatment on the IL-6 content of primary hepatic 3D cell co-cultures of chicken origin assessed by chicken-specific ELISA test. BAI and DON-treated cells were compared to the control group. Different baicalin and 2 µg/mL DON-treated cells were compared to the 2 µg/mL DON group. Different baicalin and 20 µg/mL-DON treated cells were compared to the 20 µg/mL DON group. After 24 h, the 5 µg/mL baicalin concentration elevated the IL-6 level in case of both DON treatments. Additionally, the applied 15 µg/mL baicalin also increased the amount of IL-6 compared to the 20 µg/mL DON group. After 48 h, the 15 µg/mL baicalin treatment reduced the IL-6 concentration. Control: cells without DON or BAI exposure; BAI: cells treated with BAI; DON: cells treated with DON; DON + BAI: cells treated with the combination of DON and BAI. See Table 1 for detailed concentrations. Results are expressed as mean ± SEM. *p < 0.05; **p < 0.01.