Fig. 8

Effects of (a) 24 h and (b) 48 h of deoxynivalenol (DON) and baicalin (BAI) treatment on the IFN-γ content of primary hepatic 3D cell co-cultures of chicken origin assessed by chicken-specific ELISA test. BAI and DON-treated cells were compared to the control group. Different baicalin and 2 µg/mL DON-treated cells were compared to the 2 µg/mL DON group. Different baicalin and 20 µg/mL-DON treated cells were compared to the 20 µg/mL DON group. After 24 h, the 45 µg/mL baicalin reduced, while both DON treatments increased the amount of IFN-γ. Compared to the 2 µg/mL DON group, the 15 and 45 µg/mL baicalin treatment decreased, while compared to the 20 µg/mL DON group, the 15 µg/mL increased the IFN-γ concentration. Control: cells without DON or BAI exposure; BAI: cells treated with BAI; DON: cells treated with DON; DON + BAI: cells treated with the combination of DON and BAI. See Table 1 for detailed concentrations. Results are expressed as mean ± SEM. *p < 0.05; **p < 0.01; ***p < 0.001.