Fig. 3
Social preference behavior in male and female rats is unaffected by duration and timing of isolation in the Social vs. Empty task. (A) Mean total time spent investigating a social stimulus or the empty following jSI at P21-P42 and subsequent re-housing until P60 (jSI-A). Male GH, n = 14; male jSI-A, n = 14; female GH, n = 17; female jSI-B, n = 16. (B) Mean total investigation time for the social stimulus and the empty chamber plotted in 1-min intervals over the 5-min testing period. (C) Mean bout duration representing the average length of time spent investigating the social stimulus or the empty chamber. (D-F) Data for social reward-seeking behavior observed immediately after jSI isolation at P21-P42, without rehousing (jSI-B), using the same analysis as in A-C. Male GH, n = 12; male jSI-B, n = 17; female GH, n = 13; female jSI-B, n = 10. Statistical significance indicated as ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05 based on post-hoc tests following the main effect. Error bars represent standard error of the mean (SEM).
