Fig. 2
From: Cathepsin D inhibits AGEs-induced phenotypic transformation in vascular smooth muscle cells
Overexpression of CTSD inhibits AGEs-induced proliferation and migration of VSMCs. (A) Western blot analysis of the relative expression levels of CTSD in VSMCs. (B) Immunofluorescence staining of CTSD in VSMCs (scale bar = 100 μm). (C) Western blot analysis of the relative expression levels of PCNA in VSMCs. (D) Quantification of the percentage of EdU-positive cells; blue indicates nuclear staining and red indicates EdU staining. (E) Flow cytometry analysis of cell cycle distribution, with histograms showing the percentage of cells in each phase of the cell cycle. (F) Scratch assay results of VSMCs, with histograms showing the migration rates of cells in different groups. All data are presented as mean ± SEM (n = 3). * represents p < 0.05, ** represents p < 0.01. Note: PCNA in the figure represents Proliferating Cell Nuclear Antigen.
