Fig. 7

Isolation of cone responses by ex vivo ERG recordings. (a) Control response family. Flash was given one time at 2.2 s (arrow). Flash wavelength: 500 nm. Intensity: 32–32,000 photons μm⁻². Red trace: 320 photons μm⁻². (b) Responses to paired flashes. Flashes were given two times at 0.2 and 2.2 s (arrows). First flash: 500 nm, 32,000 photons μm⁻². Second flash: same as in (a). (c) Isolated cone responses obtained by clipping 2–4 s in (b) with subsequent baseline adjustment. (d) Intensity-response relationship of cone responses. Plots were fitted with Naka-Rushton function with R_max = 72 µV, I_1/2 = 661 photons µm⁻², and n = 0.89. Plots are mean ± SEM (n = 6). The estimated cone response fitting curve in Fig. 6b is redrawn in blue for comparison to demonstrate the slight suppression of the cone responses by the conditioning first flash.