Table 2 Primer pairs used for detecting β-lactam harboring resistance genes in E. coli isolated from Sonali chicken meat.
Primer set | Gene target | β-lactamase type | Primers | Sequences (5’—3’) | Amplicon size (bp) | References |
|---|---|---|---|---|---|---|
2 | blaTEM | ESBL | TEM-F | GCGGAACCCCTATTCG | 964 | |
TEM-R | ACCAATGCTTAATCAGTGAG | |||||
blaCTX-M | ESBL | CTX-M-F | ATGTGCAGYACCAGTAARGTKATGGC | 592 | ||
CTX-M-R | TGGGTRAARTARGTSACCAGAAYSAGCGG | |||||
blaCTX-M-1 | ESBL | CTX-M-1-F | GGTTAAAAAATCACTGCGTC | 863 | ||
CTX-M-1-R | TTGGTGACGATTTTAGCCGC | |||||
blaCTX-M-2a | ESBL | CTX-M-2a-F | GATGAGACCTTCCGTCTGGA | 397 | ||
CTX-M-2a-R | CAGAAACCGTGGGTTACGAT | |||||
3 | blaSHV | ESBL | SHV-F | TTATCTCCCTGTTAGCCACC | 795 | |
SHV-R | GATTTGCTGATTTCGCTCGG | |||||
blaOXA-1 | ESBL | OXA-1-F | GGACCAGATTCAACTTTCAAG | 564 | ||
OXA-1-R | GACCCCAAGTTTCCTGTAAGTG | |||||
4 | blaCMY | AmpC | CMY-F | GACAGCCTCTTTCTCCACA | 1007 | |
CMY-R | TGGAACGAAGGCTACGTA | |||||
blaNDM-1 | MBL | NDM-1-F | CTTCCAACGGTTTGATCGTC | 465 | ||
NDM-1-R | TAGTGCTCAGTGTCGGCATC |
