Fig. 1

Subclonal molecular heterogeneity in PCOs. (a) Heatmap of pathologic alterations between pairwise tumor (black, T) and expanded PCOs (gray, O) for reported pathologic driver alterations with split designation (triangles) for multiple concurrent gene alterations. All variants colored on heatmap according to relative variant allele frequency (rVAF). All cases were mismatch repair proficient. (b) Analysis of subclonal non-synonymous variants (NSV) defined between 10–30% from cancer hotspot testing in PCOs. (c–e) Representative examples of clonality including clonal (black) and subclonal variance (gray) across representative cancers. (f) Multisite sampling from patient LR9 from endoscopic biopsy with VAF plotted including clonal (black) and discordant subclonal alterations in EXO1, MLH1, and KIT from LR9A (red) and LR9B (blue).