Fig. 1

Live cell imaging of mNGCol1α2 expressed in HT1080 cells. (A) Diagram of mNG-Col1α2 fusion protein. (B) HT1080 cells expressing mNG-Col1α2. mNG-Col1α2 highlights small rapidly moving particles which predominantly move towards the periphery of the cell. (C) Time coding projection illustrates passage of particles towards the periphery. (D) Particles were isolated and tracked using TrackMate and Fiji to calculate size, number and velocity. Scale = 10 μm, 206.44 ms per frame or 4.844 fps. (E) Combined box and whisker and beeswarm plots of HT1080 collagen vesicle diameter showing all data points. Mean diameter = 0.47, standard deviation = 0.07 n = 150 (3 replicates per cell, 10 cells per replicate, 5 vesicles per cell. (F) Combined box and whisker and beeswarm plots of HT1080 collagen vesicle speed showing all data points. Mean speed = 0.84 μms⁻¹ Standard deviation = 0.14 μms⁻¹, n = 46.