Fig. 3

mNGCol-1α2 secretion events. (A) Full field of view TIRF image of an mNG-Col1α2 expressing HT1080 cell. Red + mark locations of collagen ‘burst’ events. These events usually clustered at the periphery of the cell on the lower surface. Scale = 20 μm. (B) TIRF movie frames of a burst event. 100 ms between frames. Scale = 1 μm. (C) Kymograph of burst event derived from yellow line in B. (D) Box and whisker plot of stationary phase of vesicles at the PM before they fuse with PM to give a burst event.