Fig. 4

mNG-Col1α2 labels fast-moving carriers in Saos-2 cells with movement along microtubules. (A) mNG-Col1α2 labels collagen carriers or vesicles which rapidly move towards the periphery. These paths are shown by temporal colour coding. TrackMate tracks are used to quantify vesicle size and velocity. Scale = 10 µm. (B) Saos-2 cells expressing mNG-Col1α2 co-imaged with microtubules labelled by SiR-tubulin. Overlay shows mNG-Col1α2 association with microtubule. Superimposed on these images are the paths of TrackMate detected paths of mNG-Col1α2 carriers demonstrating their movement along microtubules. (C) Combined box and whisker and beeswarm plots of Saos-2 collagen vesicle diameter showing all data points. Mean diameter = 0.53 μm, standard deviation = 0.09 μm n = 150 (3 replicates per cell, 10 cells per replicate, 5 vesicles per cell). Combined box and whisker and beeswarm plots of Saos-2 collagen vesicle speed showing all data points. Mean speed = 0.79 μms⁻¹, standard deviation = 0.14 μms⁻¹ n = 45.