Fig. 5
From: New imaging tools reveal live cellular collagen secretion, fibril dynamics and network organisation
Superresolution microscopy of collagen fibrils containing mNG-Col1α2 deposited by Saos-2 cells. (A) Airyscan jDCV Super-resolution image of deposited mNG-Col1α2 collagen fibrils (green). Fibrils are co-labelled with anti-Collagen 1 antibody (red). In the merge panel areas of colocalisation along the fibril appear yellow. Zoomed area shows the relationship between accessible native Col1 epitopes and the incorporated mNG-Col1α2. Scale = 10 μm. (B) FWHM analysis of deposited collagen in A. Box and whisker plot shows range and of collagen diameter values. SD = 12.53 n = 32. (C) 3DSIM super-resolution imaging of deposited mNG-Col1α2 fibrils. These three-dimensional stacks reveal loops within fibrils. Scale = 5 μm.
