Fig. 9
From: Multi-omic analyses reveal PTPN6’s impact on tumor immunity across various cancers
Experimental evaluation of PTPN6 in colorectal cancer (CRC) tissues and cell lines. (A) Immunohistochemistry images showing PTPN6 protein staining in CRC tumor tissues and normal colon sections; (B) Western blots of PTPN6 protein levels across CRC cell lines (Original blots are presented in Supplementary Fig. 4); (C) Quantitation of relative PTPN6 protein levels from the Western blots in (B) using ImageJ, compared to PTPN6 mRNA expression levels from the CCLE database; (D) Western blots and qPCR validating PTPN6 knockdown efficiency by siRNAs(Original blots are presented in Supplementary Fig. 4); (E) Wound healing assay images comparing siPTPN6 and control groups at 0 h and 24 h post-scratch (red dashed lines indicate scratch); (F-G) CCK8 and plate colony formation assays assessing impacts of PTPN6 knockdown on CRC cell proliferation; (H) Immunofluorescence imaging validating subcellular distribution of PTPN6 (DAPI staining in blue, F-actin in red, PTPN6 in green). White scale bar denotes ten µM. Data availability statement. The original contributions presented in the study are included in the article. Further inquiries can be directed to the corresponding author.
