Fig. 3 | Scientific Reports

Fig. 3

From: A novel peptide RR-171 derived from human umbilical cord serum induces apoptosis and pyroptosis in pancreatic cancer cells

Fig. 3

RR-171 induces apoptosis in pancreatic cancer cells. (A) After Capan-2 and Bxpc-3 cells were treated with RR-171 for 24 h, the ratio of apoptotic cells was detected by flow cytometry using PI/Annexin V-FITC staining. *P < 0.05 and ***P < 0.001compared with the 0 µM RR-171 treatment group. (B,C) Western blotting detection of proteins related to apoptosis in Capan-2 and Bxpc-3 cells treated with 0, 5, 10, and 20 µM RR-171 for 24 h. (D) Capan-2 and Bxpc-3 cells were treated with RR-171 and Z-VAD-FMK or DMSO, and the apoptosis ratio was detected by flow cytometry using PI/Annexin V-APC double staining. **P < 0.01 and ***P < 0.001 compared with the control group.

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