Fig. 2 | Scientific Reports

Fig. 2

From: Selective activation of antioxidant resources and energy deficiency in Marinesco–Sjögren syndrome fibroblasts as an adaptive biological response to Sil1 loss

Fig. 2

Study of Nrf2 and NFkB activation in MSS fibroblasts. (A,B) Western blot showing the levels of Nrf2 (A) and NF-kB p65 (B) phosphorylation in patient-derived fibroblasts (MSS) and controls (CT). In both cases the phosphorylated form represents the transcriptionally active factor. (C) Western blot analysis of IκBα revealed a decreased protein level in MSS compared to CT cells. Statistical analysis was performed using an unpaired t-test with Welch’s correction (**P < 0.01). (D) IF analysis of the intracellular distribution of phosphorylated NF-kB p65. CT and MSS cells were plated to 70% confluency, and the following day, cells were fixed and processed for IF. NF-κB p65 staining is shown in red, and DAPI in blue. Merged images are also provided. Scale bar: 20 μm.

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