Fig. 4
TRIM6 Activates EMT and Promotes Snail1-Mediated Cell Migration in HCC. (A, B) Western blot analysis confirming the knockdown of TRIM6 in HCCLM3 (A) and Huh-7 (B) cells using two different shRNAs (sh#1 and sh#2). β-Actin was used as a loading control. (C, D) Western blot analysis showing the overexpression of TRIM6 in HCCLM3 (C) and Huh-7 (D) cells. β-Actin was used as a loading control. (E) Relative Snail1 mRNA levels in HCCLM3 and Huh-7 cells after TRIM6 knockdown (sh#1 and sh#2). TRIM6 knockdown significantly reduces Snail1 expression. ***p < 0.001. (F) Relative Snail1 mRNA levels in HCCLM3 and Huh-7 cells after TRIM6 overexpression. TRIM6 overexpression significantly increases Snail1 expression. **p < 0.01, ***p < 0.001. (G, H) Transwell migration assays showing the effect of TRIM6 knockdown on cell migration in HCCLM3 and Huh-7 cells. Knockdown of TRIM6 significantly reduces the number of migrating cells. ***p < 0.001, ****p < 0.0001. (I, J) Transwell migration assays showing the effect of TRIM6 overexpression on cell migration in HCCLM3 and Huh-7 cells. Overexpression of TRIM6 significantly increases the number of migrating cells. **p < 0.01, ***p < 0.001. These results demonstrate that TRIM6 enhances EMT and cell migration in HCC cells through the regulation of Snail1, highlighting its potential role in promoting cancer metastasis by facilitating the EMT process.
