Fig. 5 | Scientific Reports

Fig. 5

From: STAT3-mediated upregulation of TRIM6 promotes hepatocellular carcinoma invasion through the DDX58-Snail1 axis

Fig. 5

TRIM6 Interacts with DDX58. (A) Protein–protein interaction (PPI) network analysis using the BioGRID database, identifying DDX58 as a potential interacting partner of TRIM6. (B, C) Co-IP assays performed in HCCLM3 (B) and Huh-7 (C) cell lines demonstrate the physical nteraction between TRIM6 and DDX58. In (B), TRIM6 was immunoprecipitated, and the presence of DDX58 in the complex was confirmed by Western blotting. In (C), DDX58 was immunoprecipitated, and TRIM6 was detected in the complex. (D) Predicted interaction interface between TRIM6 and DDX58 based on structural modeling using Alphafold3. The zoomed-in view highlights key interacting residues on TRIM6 (ASN-409, PRO-429, and ARG-431) that recognize and bind to DDX58.

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