Fig. 5

Quantification of neutralizing titers in human serum samples. Resulting data from EMN assay for hMPV-A1 (A) and hMPV-B1 (B), then cumulative results from both strains (C). Each dot/square represents the GMT of a single sample tested in duplicate; the y axis shows 50% NT expressed as the reciprocal of the serum dilution. The lower limit of detection was set at 5, which is half of the reciprocal of the first serum dilution in plates (dashed black line). Cut-off for the calculation of the 50% NT was determined as described in Fig. 2. GMT: geometric mean titer; NT: neutralizing titer.