Table 1 Repeatability, Intermediate precision and Format Variability data for hMPV-A1 and hMPV-B1. Values expressed in percentage indicate GCV. Acceptability criteria: GCV ≤ 65.5% for Repeatability; GCV ≤ 129.0% for Intermediate precision; GCV ≤ 81.5% for Format Variability. The assay meets all criteria for both strains. GCV: geometric coefficient of variation; GMT: geometric mean titer.
hMPV-A1 | ||||||||
|---|---|---|---|---|---|---|---|---|
Fold dilution | Observed GMT | Repeatability (%) | Fold dilution | Observed GMT | Intermediate precision (%) | Fold dilution | Observed GMT | Format variability (%) |
1 | 640 | 0.0% | 1 | 640 | 0.0% | 1 | 640 | 0.0% |
2 | 302 | 22.2% | 2 | 302 | 22.2% | 2 | 302 | 15.2% |
4 | 107 | 41.4% | 4 | 107 | 43.2% | 4 | 107 | 28.9% |
8 | 63 | 39.8% | 8 | 63 | 41.4% | 8 | 63 | 27.8% |
16 | 38 | 22.2% | 16 | 38 | 22.2% | 16 | 38 | 15.2% |
32 | 19 | 22.2% | 32 | 19 | 22.2% | 32 | 19 | 15.2% |
64 | 10 | 0.0% | 64 | 10 | 0.0% | 64 | 10 | 0.0% |
hMPV-B1 | ||||||||
|---|---|---|---|---|---|---|---|---|
Fold dilution | Observed GMT | Repeatability (%) | Fold dilution | Observed GMT | Intermediate precision (%) | Fold dilution | Observed GMT | Format variability (%) |
1 | 718 | 22.2% | 1 | 718 | 56.4% | 1 | 718 | 37.2% |
2 | 339 | 22.2% | 2 | 339 | 22.2% | 2 | 339 | 15.2% |
4 | 170 | 22.2% | 4 | 170 | 22.2% | 4 | 170 | 15.2% |
8 | 85 | 41.4% | 8 | 85 | 43.2% | 8 | 85 | 28.9% |
16 | 42 | 22.2% | 16 | 42 | 22.2% | 16 | 42 | 15.2% |
32 | 21 | 32.7% | 32 | 21 | 46.7% | 32 | 21 | 31.1% |
64 | 13 | 22.2% | 64 | 13 | 46.7% | 64 | 13 | 31.1% |
