Fig. 7
From: Role of alpha-1 antitrypsin in Bruch’s membrane integrity
AMD patients exhibit lower AAT expression levels in the choroid. (a) Immunohistochemistry (IHC) showing decreased AAT protein levels in the BrM/Choroid layer (yellow arrows) in donor eyes of both, GA (Grade 4) and CNV (Grade 5B) patients (bottom row) when compared to age-matched non-diseased donor retinas (top row; Scale bar: 50 μm; ). Sections from three different donors per group were analyzed. (b) AAT mRNA expression (red dotted signal: yellow arrows) is detected in the choroid by RNAscope in non-diseased donor eyes (top; Scale bar: 20 μm), while no signal is seen in AMD patients. Two of the three patients shown in (a) are shown in (b). Age in years, gender of donor eyes, and disease stage where applicable, is indicated in each panel (Chrd, choroid; ONL, outer nuclear layer; yrs: years; F: female).
