Fig. 5
PCR verification of recombination products associated with the repetitive sequences (R01, R04, and R07) on MC1 and MC2. (A) Schematic representation of the four configurations (c1–c4) associated with each repetitive sequence. The regions corresponding to the primers are shown as red blocks. f1 and f2: forward primers. r1 and r2: reverse primers. (B) Electrophoretic gel plot of PCR products amplified with various combinations of forward and reverse primers to amplify the DNA molecules corresponding to configurations c1–c4. The name of the repetitive sequence, combinations of forward and reverse primers, expected configuration to be amplified, and the lane numbers are shown above the gel plot.
