Fig. 3 | Scientific Reports

Fig. 3

From: Single-cell RNA sequencing characterization of Holstein cattle blood and milk immune cells during a chronic Staphylococcus aureus mastitis infection

Fig. 3

Similar cell type proportions recovered from milk and blood via single-cell RNA sequencing and flow cytometry. (A) Stacked bar plot showing the proportion of cells (y-axis) comprising each sample used for scRNA-seq (x-axis). Fill color of bars corresponds to cell type. Bar size is proportional to 100% of cells comprising a sample and is not proportional to the total absolute number of cells in each sample. (B) Comparative cell type percentages as indicated by flow cytometry analysis. Percentages are relative to CD45 + live single cells with the exception of CD3 + cells whose percentages are within CD45 + live single cell lymphocytes (black arrow). ASC antibody-secreting cell, cDC conventional dendritic cell, ILC innate lymphoid cell, logFC log fold change, pDC plasmacytoid dendritic cell, scRNA-seq single-cell RNA sequencing.

Back to article page