Fig. 1

(A) Comparative analysis of drug sensitivity between MV4-11 naive and SYKi-resistant cell lines. Scatter plot showing the correlation of AUC (Area Under the dose-response Curve) values for all compounds from the NCATS screening library tested in MV4-11 naive versus resistant cell lines. Each point represents an individual compound, with color intensity and size reflecting potency and differential response (where a stronger red represents greater differential killing between the two cell lines). The black diagonal line represents y = x (equal response in both cell lines), while additional solid and dashed lines indicate ± 0.5 standard deviations (SD) and ± SD from the diagonal. Lower AUC values indicate more potent and efficacious compounds. (B) Annotated version of the scatter plot displaying all tested glucocorticoid compounds with differences in AUC between the naive and resistant cell lines. Data points are labeled with the drug/compound names, with those above or below the y = x line indicating potential resistance or hypersensitivity in the resistant MV4-11 cells. Viability analysis using an ATP-based luminescent assay with increasing concentrations of dexamethasone (C) and prednisolone (D) in MV4-11 naive and SYKi resistant cells.