Fig. 3
From: Inflammatory signaling pathways play a role in SYK inhibitor resistant AML
(A) Immunoblot confirming overexpression of IL3 ORF in MV4-11 cells. Vinculin was used as a loading control. Representative images of three independent replicates are shown. Original Blots are presented in Supplementary Fig. 4. (B) Long-term viability assay in MV4-11 (left) and MOLM14 (right) cells overexpressing the indicated ORFs and treated with vehicle or 3 µmol/L entospletinib (Ento) (****P ≤ 0.0001, two-way ANOVA test, multiple comparisons) (C) Colony forming assay in MV4-11 (left) and MOLM14 (right) cells overexpressing the indicated ORFs and treated with increasing concentrations of entospletinib (Ento). (*P ≤ 0.05, ****P ≤ 0.0001, two-way ANOVA test, multiple comparisons) (D) Colony forming assay with vehicle or IL3 addition (10 ng/ml) to methylcellulose in MV4-11 (left) and MOLM14 (right) cells treated with increasing concentrations of entospletinib (****P ≤ 0.0001, two-way ANOVA test, multiple comparisons).
