Fig. 2
The combination index (CI) analysis which obtained from Compusyn confirms that the combination of AsA and X-radiation exhibits a synergistic effect, suggesting potential therapeutic benefits for THP-1 and K562 cell treatment. (A) The Dose–Effect Curve of THP1cells, the combination index (CI) values for low (2.5 µg/mL) and high (5 µg/mL) concentrations of AsA combined with X- radiation at doses 2, 4, and 8 Gy for THP-1 cells. (B) Dose–Effect Curve of K562 cells, specifically pertain to the CI values of K562 cells, low doses of AsA (4 µg/mL) and high (8 µg/mL) highlighting the interaction between AsA and different doses of ionizing radiation. (C and D) Combination Index (CI) plot for the interaction between AsA and X-radiation in THP-1 and K562 cells, respectively. The CI values are plotted against the fraction affected (Fa). Data points below CI = 1 indicate a synergistic effect, suggesting that the combination treatment enhances cytotoxicity compared to individual treatments. (E and F) Isobologram curve of the combination between AsA and X-radiation in leukemia cells (THP-1 and K562) respectively. The diagonal line represents the additive effect, while data points below the line indicate synergy and points above the line indicate antagonism. The observed distribution of data suggests a synergistic interaction between AsA and X-radiation, supporting their potential combined therapeutic efficacy. The strongest synergy was detected at higher radiation dose like 8 Gy which combined with AsA, suggesting enhanced therapeutic potential.
