Fig. 3 | Scientific Reports

Fig. 3

From: The potentially therapeutic effects of ascorbic acid in different cell line in attempt to reduce the risk of radiation therapy

Fig. 3Fig. 3

Flow cytometric analysis of Ascorbic Acid (AsA) and X-irradiation-induced apoptosis in THP-1 cells. (A) and (C) Representative flow cytometry plots using Annexin V-AF 488/PI staining for apoptosis for THP-1 & K562 cells, respectively. All cells were treated for 24 h and then stained with Annexin V-AF 488 and PI for flow cytometric analysis. Quadrant Q1 represents the percentage of viable cells. Quadrants Q2 and Q3 represent the percentages of cells in the early and late apoptotic phases, respectively. Quadrant Q4 represents the percentage of dead cells. (B) and (D) The bar chart shows the percentage of THP-1%K562 cell, respectively in each apoptotic stage. Data are presented as the mean ± standard deviation. Statistical significance is indicated as follows: ***p < 0.001, **p < 0.01, *p < 0.05.

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