Fig. 8
SPIB contributes to AST by promoting SAA1 transcription. (A) The distribution and annotations of single cells labelled by cluster identity. (B,C) Expression profiles of SPIB and SAA1 for each cell based the single-cell transcriptome sequencing data. (D) HE staining of ccRCC tissues corresponding to the spatial transcriptomics results. (E,F) Expression profiles of SPIB and SAA1 for each cell based the spatial transcriptome sequencing data. (G) The expression of SAA1 between ccRCC tissues and normal tissues using western blotting (5 pairs). (H) Representative images of SAA1 immunohistochemistry. (I) The expression of SAA1 between ccRCC tissues and normal tissues using RT-qPCR (5 pairs). (J) Expression of SAA1, phosphorylated AKT, AKT, phosphorylated mTOR, mTOR, β-actin detected after transfection with siRNA in Caki-1 cell lines using western blotting. (K) Representative cell images of si-NC and si-SAA1 cells in culture plate and media. Original blots are presented in Supplementary Fig. 2.
