Fig. 5
Treatment with Mg ions stimulates mineralization of human adipose-derived stem cells (ADSCs). ADSCs were cultured in osteogenic medium in 24-well plates (2.0 × 104 cells per well) in the presence or absence of 2.5 or 10 mMOL Mg2+. Culture medium was changed every 4 days. Alizarin Red S staining was performed on days 13 or 20, and cells were observed microscopically (A). Quantification of Alizarin Red S stain extracted in 5% formic acid in culture wells, based on absorbance at 450 nm using an iMark Microplate Absorbance Reader (BIO-RAD). Values were normalized to those in untreated samples. Data represents mean relative absorbance ± SD at day 13 (B) or 20 (C) (each n = 3, *p < 0.05; **p < 0.01).
