Fig. 3

Example of segmentation of trabecular meshwork (TM) in a normal eye showing the (A) original image, (B) segmentation overlain (blue – semi-automated, red – manual 1, green – manual 2) demonstrating the variability in the TM segmentation between the 2 manual segmentations as well as the semi-automated segmentation. (C) Diagram illustrating the ImageJ local thickness measurement as the average diameter of the largest circles (examples in red circles) that fit within the object at any point.