Fig. 1

CYB5A expression during osteogenic differentiation of MC3T3-E1 cells. (A) qRT-PCR analysis showed that CYB5A mRNA expression was significantly upregulated during MC3T3-E1 cell osteogenic differentiation. (B) Western blot analysis and densitometric quantification confirmed that CYB5A protein levels increased during osteogenic differentiation. β-actin served as an internal control for normalization (n = 3). Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001.