Fig. 2
CYB5A did not affect the proliferation of MC3T3-E1 cells but promoted their migration. (A&G) qRT-PCR analysis confirmed the efficiency of CYB5A overexpression via plasmid and knockdown via siRNA in MC3T3-E1 cells. (B&H) Western Blotting analysis and quantification verified the effectiveness of CYB5A overexpression and knockdown at the protein level. (C&I) The cell viability of MC3T3-E1 cells was assessed using CCK‑8 assay. (D&J) Cell proliferation activity of MC3T3-E1 cells was evaluated using EdU assay, and the proportion of EdU-positive cells was calculated (ns = no significant difference, scale bar = 100 μm). (E&K) MC3T3-E1 cell migration was examined using the Transwell assay, and the number of migrated cells was quantified (scale bar = 100 μm). (F&L) The migration ability of MC3T3-E1 cells was analyzed using scratch assay, and the migration efficiency was calculated (scale bar = 500 μm). β-actin served as the internal control for normalization. Data are expressed as mean ± standard deviation (n = 3). Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
