Fig. 5 | Scientific Reports

Fig. 5

From: CYB5A promotes osteogenic differentiation of MC3T3-E1 cells through autophagy mediated by the AKT/mTOR/ULK1 signaling pathway

Fig. 5

The effects of autophagy inhibitors and activators, as well as lysosomal inhibitors, on osteogenesis and autophagy in MC3T3-E1 cells. (A&D&I) Western Blotting and quantification showed changes in osteogenic marker (COL1A1, ALP, RUNX2, and OPN) expression. (B&E) ARS staining of MC3T3-E1 cells after osteogenic induction for 21 days (scale bar: 500 μm). (C&F) ALP staining of MC3T3-E1 cells after osteogenic induction for 14 days (scale bar: 500 μm). (D&H&J) Western blotting and quantification of levels of LC3-I/II and P62 in MC3T3-E1 cells. β-actin levels served as the internal normalization control. Data are presented as mean ± standard deviation (n = 3). ns = no significant difference. Statistical significance: *p < 0.05, **p < 0.01, ***p < 0.001.

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