Table 2 PCR primers designed in this study.
Serotype | Target gene | Primer name | Sequence (5‘—3’) | Product size |
|---|---|---|---|---|
A1 | glycosyltransferase | Mh_A1_F | TCCCCTGTTGCAAATACACC | 990 bp |
| Â | Â | Mh_A1_R | TACTTCACGTATGCCCAGCA | Â |
A2 | hypothetical protein | Mh_A2_F | GGAACAGGGGCTTTGTATCT | 332 bp |
| Â | Â | Mh_A2_R | GTTGCATGCCCAGAATAAGG | Â |
A5 | glycosyltransferase | Mh_A5_F | ATGTGCGAGAGAGGTTGCTT | 408 bp |
| Â | Â | Mh_A5_R | CGTCACATTGCACCTCAGAT | Â |
A6 | glycosyltransferase | Mh_A6_F | GGCATGCGGTACACCTGTAAT | 250 bp |
| Â | Â | Mh_A6_R | CAATGGGGCTTCAGGCTTA | Â |
A7 | glycosyltransferase | Mh_A7_F | ATGGTGGGCTCTCAAGTTCA | 307 bp |
| Â | Â | Mh_A7_R | TATACTGCTGTTGCCGCAGA | Â |
A8 | glycosyltransferase | Mh_A8_F | GGTGTATCTCATGCGGTTGGA | 429 bp |
| Â | Â | Mh_A8_R | GCTTTGCGTTGAAGAGGCT | Â |
A9 | hypothetical protein | Mh_A9_F | GCCTTCCAATTTTGGAGCTG | 760 bp |
| Â | Â | Mh_A9_R | GCCTCCACCAAGTATACGAA | Â |
A12 | glycosyltransferase | Mh_A12_F | ATACTTCCGGTTCTGCTGGA | 169 bp |
| Â | Â | Mh_A12_R | CCCAGAAATGGGCTATCACT | Â |
A13 | CDP-glycerol glycerophosphotransferase | Mh_A13_F | CTCATGATGATGGGGGATAC | 221 bp |
| Â | Â | Mh_A13_R | TGCCCTGTATTAGGGTGAGA | Â |
A14/A16 | glycosyltransferase | Mh_A14/A16_F | GTGCTTATTGGTCTGCAGGT | 132 bp |
| Â | Â | Mh_A14/A16_R | CACCATAAGCTCTTGCTGGA | Â |
A17 | glycosyltransferase | Mh_A17_F | AAGGGCAGGTTAAAGCACCT | 791 bp |
| Â | Â | Mh_A17_R | TCTTGCTACCTGCCTTTGCT | Â |
- | recN (Mh-specific sequence) | Mh-spe_recN_F | CCTTTACTATGCAGCCACAT | 591 bp |
| Â | Â | Mh-spe_recN_R | GGCTATCAACCTCAGCCAAG | Â |
