Fig. 3 | Scientific Reports

Fig. 3

From: Mipep deficiency in adipocytes impairs mitochondrial protein maturation and leads to systemic inflammation and metabolic dysfunctions

Fig. 3

Adipocyte-specific Mipep knockout (aMKO) in mice increases inflammatory-related gene expression and decreases lipid metabolism-related gene expression in WAT. Expression analysis using RNA-seq of gWAT and BAT from 27-week-old control and aMKO mice. Four control and aMKO mice from each group were used as experimental replicates. (A and B) GO enrichment analysis using DAVID identified significantly enriched gene sets among DEGs from RNA-seq data of gWAT from aMKO mice compared with control mice. Up-regulated genes (log 2 FC ≥ 1)    (A) and down-regulated genes (log 2 FC ≤ − 1) (B). (C) The volcano plot presents DEGs in the gWAT of aMKO mice compared with control mice from four samples (total = 15,815 variables). Red indicates up-regulated genes (log 2 FC ≥ 1) and blue indicates down-regulated genes (log 2 FC ≤ − 1); the log10 p-value threshold was p > 5. (D and E) GO enrichment analysis using DAVID identified significantly enriched gene sets among DEGs from RNA-seq data of BAT from aMKO mice compared with control mice. Up-regulated genes (log 2 FC ≥ 1)   (D) and down-regulated genes (log 2 FC ≤ − 1) (E). (F) The volcano plot presents DEGs in the BAT of aMKO mice compared with control mice from four samples (total = 14,420 variables). Red indicates up-regulated genes (log 2 FC ≥ 1) and blue indicates down-regulated genes (log 2 FC ≤ − 1); the log10 p-value threshold was p > 5. BAT, brown adipose tissue; BP, biological process; DAVID, Database for Annotation, Visualization, and Integrated Discovery; FC, fold change; gWAT, gonadal white adipose tissue.

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