Fig. 4 | Scientific Reports

Fig. 4

From: Mipep deficiency in adipocytes impairs mitochondrial protein maturation and leads to systemic inflammation and metabolic dysfunctions

Fig. 4

Mipep depletion in both WAT and BAT decreases adipocyte differentiation and mitochondrial biogenesis-related genes and mtDNA copy number. mRNA and mtDNA levels in the gWAT (A and B) and BAT (C and D) of 27-week-old control and aMKO mice obtained by real-time PCR. (A) mRNA expression levels of adipocyte differentiation, mature adipocyte, and mitochondrial biogenesis genes in gWAT. (B) mtDNA copy number in gWAT. (C) mRNA expression levels of adipocyte differentiation, mature adipocyte, and mitochondrial biogenesis genes in BAT. (D) mtDNA copy number of BAT. Each dot represents one mouse. Real-time PCR data were normalized to Rps18 expression for measurement of mRNA levels and Hprt1 expression for measurement of mtDNA levels. Values are mean ± SD. Differences between values were analyzed by Student’s t-test. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control. BAT, brown adipose tissue; gWAT, gonadal white adipose tissue; mtDNA, mitochondrial DNA.

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