Fig. 5 | Scientific Reports

Fig. 5

From: Mipep deficiency in adipocytes impairs mitochondrial protein maturation and leads to systemic inflammation and metabolic dysfunctions

Fig. 5

Adipocyte-specific Mipep knockout (aMKO) in mice differentially increases the mitochondrial stress response in WAT and BAT. The intracellular stress response of gWAT (A–E) and BAT (F–J) from 27-week-old control and aMKO mice. (A–D) mRNA expression levels of mitokine (A), chaperone and protease (B), endoplasmic reticulum stress-related (C), and stress response-related transcriptional factor genes (D) in gWAT. (E) The ratio of phospho-eIF2α (Ser51)/total-eIF2α protein expression in gWAT. (F–I) mRNA expression levels of mitokine (F), chaperone and protease (G), endoplasmic reticulum stress-related (H), and stress response-related transcriptional factor genes (I) in BAT. (J) The ratio of phospho-eIF2α (Ser51)/total-eIF2α protein expression in BAT. Real-time PCR data were normalized to Rps18 expression for measurement of mRNA levels. Each dot represents one mouse. Values are mean ± SD. Differences between values were analyzed by Student’s t-test. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control. BAT, brown adipose tissue; gWAT, gonadal white adipose tissue; eIF2α, eukaryotic translation initiation factor 2 A.

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