Fig. 6

Macrophage infiltration is increased in WAT from adipocyte-specific Mipep knockout (aMKO) mice. Macrophage-related inflammation of gWAT (A–E) from 27-week-old control and aMKO mice. (A) Representative flow cytometry dot plot showing the myeloid cell landscape in gWAT from control (upper) and aMKO (lower) mice. The left panel shows the F4/80 versus CD11b staining profile gated by CD45⁺ cells. The right panel shows the CD11c versus MHCII staining profile gated by CD45⁺ CD11b⁺ F4/80 ⁺ cells. (B–E) Absolute numbers of CD11b⁺ F4/40⁺ macrophages (B), MHCII^high/low CD11c^low-dominant ATMs (C,D), and obesity-related MHCII^high CD11c^high infiltrating ATMs (E) in gWAT. (F) mRNA expression levels of inflammation-related genes in gWAT. Real-time PCR data were normalized to Rps18 expression. Each dot represents one mouse. Values are mean ± SD. Differences between values were analyzed by Student’s t-test. *p < 0.05, **p < 0.01 and ***p < 0.001 vs. control. ATM, adipose tissue macrophage; CD, cluster of differentiation; gWAT, gonadal white adipose tissue; MHC, major histocompatibility complex.