Fig. 7
From: A tardive dyskinesia drug target VMAT-2 participates in neuronal process elongation
Hesperetin recovers decreased Akt phosphorylation under various experimental conditions. (A, B) Following the induction of differentiation, N1E-115 cells were treated with reserpine and hesperetin (Hes) or its vehicle and cultured for 3 days. The immuneprecipitates using antibodies against phosphorylated Akt (pAkt) and the lysates of cells were used for immunoblotting with antibodies against total Akt (Akt). The levels of Akt immunoprecipitated with an anti-phosphorylated Akt antibody were normalized to those of total Akt or actin and graphically represented (** p < 0.01; n = 3 blots). The immunoreactive levels of Akt were also normalized to those of actin. (C, D) Following the induction of differentiation, cells were treated with tetrabenazine and hesperetin (Hes) or its vehicle. The levels of phosphorylated Akt immunoprecipitates were normalized to those of total Akt or actin and graphically represented (** p < 0.01; n = 3 blots). The immunoreactive levels of Akt were also normalized to those of actin. (E, F) Cells were transfected with plasmids encoding Cas13 with and VMAT-2 one (gVMAT-2). Following the induction of differentiation, cells were treated with or without hesperetin (Hes). The levels of phosphorylated Akt immunoprecipitates were normalized to those of total Akt or actin and graphically represented (** p < 0.01; n = 3 blots). The immunoreactive levels of Akt were also normalized to those of actin.
