Table 1 Key materials used in this study.
From: A tardive dyskinesia drug target VMAT-2 participates in neuronal process elongation
Reagents or sources | Company or source | Cat. no | Lot. no | Concentration used |
|---|---|---|---|---|
Antibodies | ||||
 Anti-growth-associated protein 43 (GAP43) | Santa Cruz Biotechnology (Santa Cruz, CA, USA) | sc-17790 | J1723 | Immunoblotting (IB), 1:5,000 |
 Anti-Tau | Santa Cruz Biotechnology | sc-21796 | G1222 | IB, 1:500 |
 Akt1 antibody | CST (Danvers, MA, USA) | 2938S | 002 | IB, 1:1,000 |
 Phospho-Akt1 (phospho-Ser-473) antibody (for active phosphorylated form detection) | CST | 9018S | 002 | Immunoprecipitation (IP), 1 µg per 800 µg of proteins |
 Ant-vesicular monoamine transporter 2 (VMAT-2) | Santa Cruz Biotechnology | sc-374079 | H1022 | IB, 1:500 |
 Anti-actin (also called pan-beta type actin) | MBL (Tokyo, Japan) | M177-3 | 008 | IB, 1:5,000 |
 Anti-IgG (H + L chain) (Mouse) pAb-HRP | MBL | 330 | 365 | IB, 1:5,000 |
 Anti-Mouse IgG (Goat), HRP-conjugated, Pre-absorbed | Nacalai Tesque (Kyoto, Japan) | 21,860–61 | L3E2991 | IB, 1:10,000 |
 Anti-IgG (H + L chain) (Rabbit) pAb-HRP | MBL | 458 | 354 | IB, 1:5,000 |
Cells used | ||||
 N1E-115 | Japan Health Science Foundation (Tokyo, Japan) | N/A | N/A | 1,000,000 cells per 6-cm-dish culture |
Chemicals | ||||
 Reserpine | Cayman Chemical (Ann Arbor, Michigan, USA) | 16,474 | 0,603,016–13 | 15 micromolar concentration |
 Tetrabenazine | Cayman Chemical | 20,380 | 0,490,320–28 | 60 micromolar concentration |
 Hesperetin | Santa Cruz Biotechnology | sc-202647 | D1921 | 10 micromolar concentration |
Plasmids and vectors | ||||
 CasRx (NLS-RfxCas13d-NLS) | Addgene (Watertown, MA, USA) | 109,049 | N/A | 50 nM per one transfection |
 pEGFP-C1 | Takara Bio (Shiga, Japan; discontinued in 2005) | N/A | N/A | 50 nM per one transfection |
gRNA sequences (5' to 3’) inserted into the plasmid (pSINmU6) | ||||
 Sense chain for gLuciferase-105th (gControl) gatccGCACCCGTGCAAAAATGCAGGGGTCTAAAAC-ATCCTCTAGAGGATAGAATGGCTTTTTT at Antisense chain for gLuciferase-105th (gControl) cgatAAAAAAGCCATTCTATCCTCTAGAGGATGTTTTAGAC-CCCTGCATTTTTGCACGGGTGCg | This manuscript | N/A | N/A | 50 nM per one transfection |
 Sense chain for gVMAT-2–387 GATCCGCACCCGTGCAAAAATGCAGGGGTCTAAAACGCAAGTTGGTCTGTTGTTTGCCTTTTTTAT Antisense chain for gVMAT-2–387 CGATAAAAAAGGCAAACAACAGACCAACTTGCGTTTTAGACCCCTGCATTTTTGCACGGGTGCG | This manuscript | N/A | N/A | 50 nM per one transfection |
 Sense chain for gVMAT-2–787 GATCCGCACCCGTGCAAAAATGCAGGGGTCTAAAACGGAGCTATTCAGCTCTTTGTGCTTTTTTAT Antisense chain for gVMAT-2–787 CGATAAAAAAGCACAAAGAGCTGAATAGCTCCGTTTTAGACCCCTGCATTTTTGCACGGGTGCG | This manuscript | N/A | N/A | 50 nM per one transfection |
