Fig. 3
DA-BMSCs significantly ameliorated the behavioral deficits of a rat model of Parkinson’s disease. (A) A flow chart depicting the experimental process containing animal groups and the number of animals in each group. (B) 6-Hydroxydopamine and DA-BMSCs were stereotactically transplanted into the right medial forebrain bundle (MFB) of each model rat at the two coordinates labeled on the coronal and sagittal planes. (C) The PD rats were immobilized on the stereotaxic apparatus, and the cells were injected into the right MFB of the brain. (D) Rota-rod test on the Rota-rod apparatus. (E) APO-induced rotation test. (F) Heat maps of motion trajectory for the open field test. F1: the model group, F2: the BMSCs group, F3: the DA-BMSCs group, and F4: the control group. (G) Behavioral evaluation of the rats in the Rota-rod test. (H) Behavioral evaluation of the rats in the APO-induced rotation test. (I) Behavioral evaluation of the rats in the open field test. All data were presented as mean ± SEM. The p-values are from multiple comparisons in two-way ANOVA with Sidak’s multiple comparative tests (n = 6), *P < 0.05, **P < 0.01, ****P < 0.0001, n.s. no significance: P > 0.05.
