Fig. 8
Single-cell sequencing analysis. (A) UMAP showing seven cell types (macrophages, fibroblasts, B cells, T cells, epithelial cells, tissue stem cells, endothelial cells) based on CellMarker expression. High-risk groups have more macrophages and fibroblasts, while low-risk groups show higher levels of T cells, B cells, and epithelial cells. (B) Cell-type proportion comparison between high-risk and low-risk groups. High-risk groups exhibit increased epithelial cells and fibroblasts, with decreased T and B cells. (C) Cell composition across normal tissue, primary tumors, and liver metastases. Macrophages decrease progressively, fibroblasts are highest in primary tumors, and B cells are lowest in tumors and metastases. (D) Cell-type proportion across normal tissue, primary tumors, and liver metastases. (E,F) Riskscore increases progressively from normal tissue to primary tumors and liver metastases, with macrophage riskscores following a similar trend.
