Fig. 1
T transfection prior to standard cardiac differentiation instills sustained upregulation of cardiac transcription factors. (A) Induced pluripotent stem cells undergoing standard differentiation with and without T transfection exhibited similar beating areas. Wnt modulation was required for maximal beating area. T + CHIR denotes cells transfected with T that undergo biphasic Wnt modulation for cardiac differentiation. CHIR denotes untransfected cells that are differentiated with Wnt modulation. T indicates cells transfected with T but not exposed to standard differentiation. (B) Brachyury expression in T transfected cells was increased within 24 h of transfection and remained upregulated compared to untransfected cells. (C) Maximum upregulation of NKX2.5 in transfected cells versus untransfected cells was observed upon mesoderm induction with CHIR. (D) GATA4 expression showed an increase in transfected cells during culture with CHIR and peaked with subsequent Wnt inhibition. (E) TBX20 expression was bolstered in mesoderm potentiated cells. (F) MEF2C expression in T transfected cells was observed following upregulation of other cardiac transcription factors. Error bars represent standard deviation. (B-F) Fold changes compare T transfected cells to untransfected cells at each corresponding day of differentiation. *p < 0.05, **p < 0.01, ***p < 0.001.
