Fig. 3 | Scientific Reports

Fig. 3

From: Analytical validation of total mucin concentration assay using SEC MALLS dRI for diagnosing and monitoring mucoobstructive lung diseases

Fig. 3

Specificity and interference assessment for total mucin quantification by SEC–MALLS-dRI: (A) Effect of saliva contamination on mucin quantification. Minimal interference was observed at saliva concentrations up to 20%, with recovery within acceptable limits. Significant deviations were noted at saliva concentrations of 50%. (B) Effect of blood contamination on mucin quantification. No significant interference was observed at blood concentrations ≤ 1%. However, interference increased progressively at concentrations ≥ 2%, exceeding acceptable limits (≥ 10%) at 2% and 5%. (C) Effect of DNA contamination and mitigation with DNase treatment. DNA concentrations ≤ 1% caused no interference, but significant deviations were observed at concentrations ≥ 2%. (D) DNase treatment effectively restored mucin measurement accuracy to within acceptable thresholds, eliminating interference. LSB = Light Scattering Buffer.

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