Fig. 1
Comparison of the inner ear cell tropism of four AAV serotypes at the embryonic stage. Confocal images of the apical turn of the organ of Corti (left panels) and vestibular organs (right panels) after the injection into the otocyst on E13-E15 of 1 µL of (A) AAV2-CBA-GFP, (B) AAV8-CMV-GFP, (C) AAV-Anc80L65-CMV-GFP or (D) AAV9-PHP.eB-CBA-GFP and immunostaining on P8 for GFP (green) and myosin 7a (red). For the cochlea, the insets are close-up views of the apical (top), medial (middle), and basal (bottom) regions (scale bars: A-D, 100 μm; insets, 5 μm). IHCs, inner hair cells; OHCs, outer hair cells; SGNs, spiral ganglion neurons; DC, Deiters’ cells; NF, nerve fibers; IPhC, inner phalangeal cells, and HC, Hensen’s cells. The right panel shows a low magnification of the macula and ampulla cristae with close-up views shown in the insets (scale bars A-D, 50 μm; insets, 5 μm). GFP-positive vestibular hair cells are outlined by a dashed line and cells other than the sensory hair cells are indicated by arrowheads. (E) Bar graphs showing the percentage of hair cells—IHCs, OHCs and VHCs—transduced with AAV2 (black), AAV8 (pink), Anc80L65 (blue), and AAV9-PHP.eB (purple), (mean percentage ± SEM). Two-way ANOVA, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
