Fig. 2
Comparison of the inner ear cell tropism of four AAV serotypes at the neonatal stage. Confocal images of the apical turn of the organ of Corti (left panels) and vestibular organs (right panels) after the injection on P2, via the RWM, of 2 µL of (A) AAV2-CBA-GFP, (B) AAV8-CMV-GFP, (C) AAV-Anc80L65-CMV-GFP or (D) AAV9-PHP.eB-CBA-GFP, followed by immunostaining on P8 for GFP (green) and myosin 7a (red). For the cochlea (left), insets are high-magnification views of the apical (top), medial (middle), and basal (bottom) regions (scale bars: A–D, 100 μm; insets, 5 μm). IHCs, inner hair cells; OHCs, outer hair cells; IPC, inner pillar cells, DC, Deiters’ cells; IPhC, inner phalangeal cells. For the vestibule (right), insets are close-up views of the macula and ampulla cristae (scale bars A–D, 50 μm; insets, 5 μm). GFP-positive vestibular hair cells are outlined by dashed lines and cells other than sensory hair cells are indicated by arrowheads. (E) Bar graphs showing the percentage of hair cells—IHCs, OHCs and VHCs—transduced with AAV2 (black), AAV8 (pink), Anc80L65 (blue), and AAV9-PHP.eB (purple), (mean percentage ± SEM). Two-way ANOVA, **P < 0.01, ***P < 0.001 and ****P < 0.0001.
